E-commerce enterprise performance was one of the important issues to be considered when they developed to a certain stage, and it was realistic to research enterprise performance. SUNING was taken as an example, the typical E-commerce enterprise, 10 key factors were proposed that affected the performance and converted them into 10 key indicators to study the performance of the E-commerce enterprises. By SPSS19.0 software, the correlation test, descriptive statistics and regression analysis were made based on the 10 indicators, and the conclusion showed that the key factors influenced the E-business enterprise performance. The research conclusion would play a guiding role in the future development of E-commerce enterprises.